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This original species-specific pattern is immediately compared with reference spectra in the MALDI Biotyper library. Furthermore, the MALDI Biotyper supports the Open Microbiology Concept which allows customers to generate their own database entries from regional isolates via a push-button storage space in a customer-particular sub-library. The new second edition of the MBT Mycobacteria Library adds another 140 isolates from 37 new species. With these added features the MALDI Biotyper addresses now a lot more than 130 species of mycobacteria. The MBT Pilot is used for light-guided manual focus on planning using cross hairs to point another position for planning on the MALDI target. Barcoding of the MALDI target and the sample, along with multiple isolate support, make sure that the complete procedure is traceable fully.